Banca de DEFESA: LAYS LAIANNY AMARO BEZERRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LAYS LAIANNY AMARO BEZERRA
DATE: 04/04/2023
TIME: 09:00
LOCAL: PLATAFORMA VIRTUAL GOOGLE MEET
TITLE:

Use of essential oils from plants from the Araripe National Forest in the control from the black bean aphid Aphis craccivora Koch (Hemiptera: Aphididae)

KEY WORDS:

Alternative method. Cowpea. Aphids.

PAGES: 70
BIG AREA: Outra
AREA: Ciências Ambientais
SUMMARY:

The black aphid Aphis craccivora Koch 1854 is one of the main pests of cowpea Vigna unguiculata (L.) Walp. Due to the need to find effective and safe methods of control, research has been increasing looking for natural alternatives, such as the use of essential oils with insecticidal properties. Thus, the objective of this work was to evaluate the potential of the medicinal oils of jatobá Hymenaea courbaril, copaiba Copaifera langsdorffii and mastic Schinus terebinthifolius to control nymphs and adults of A. craccivora. The biotests were conducted in two stages: the first at the Agricultural Entomology Laboratory of the Federal University of Cariri (UFCA), in Crato-CE and the second in a greenhouse at Embrapa Algodão, in Barbalha. The oils were extracted from the leaves by the hydrodistillation method in a Clevenger-type distiller. The treatments of the laboratory tests consisted of the essential oils of the plants at 0.1%, + distilled water and the solvent Dimethylsulfoxide 2% (DMSO), in addition to the control with distilled water + the solvent. Each treatment had four repetitions, each consisting of a cowpea leaf kept inside a Petri dish containing moistened cotton, where 10 insects were placed and sprays of 0.5 mL of the respective oils were made. The plates remained in B.O.D at a temperature of 25°C ± 2°C, relative humidity of 70 ± 10% and photophase of 12 hours, and after 24, 48, 72, 96 and 120 hours of exposure, readings in the treatments considering dead the insects that did not react to a mechanical stimulus with a brush with fine bristles. Tests were initially performed with insect nymphs and subsequently with wingless adults. The tests in the greenhouse consisted of V. unguiculata plants 30 days after sowing. The treatment used was the one that obtained the best result in laboratory tests (mastic oil). Exposure to oils and treatment readings occurred as described for laboratory analysis. The data obtained were submitted to analysis of variance, regression analysis and mortality efficiency. The results for the first phase of the experiment show that mastic oil was the most efficient, with 83.33% and 75.75% mortality efficiency in nymphs and adults, respectively, after 120 hours of exposure. In tests in a greenhouse, this same oil showed 73.52% and 62.85% for nymphs and adults, respectively, opening up new perspectives regarding its use as a natural insecticide to control the black bean aphid. The α-pinene component found in mastic oil is the major component, therefore, there is a need for further studies with it alone.

BANKING MEMBERS:
Presidente - FRANCISCO ROBERTO DE AZEVEDO
Interno - FRANCISCO JOSE DE PAULA FILHO
Externo à Instituição - Walter Santos Evangelista Júnior