Banca de QUALIFICAÇÃO: JAILSON RENATO DE LIMA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JAILSON RENATO DE LIMA SILVA
DATE: 18/10/2023
TIME: 14:00
LOCAL: PLATAFORMA GOOGLE MEET
TITLE:

BIOINSETICIDE POTENTIAL OF Sarcomphalus joazeiro MART. ABOUT DISEASE VECTORS Periplaneta americana (BLATTARIA: BLATTIDAE) AND Aedes aegypti (DIPTERA: CULICIDAE)

KEY WORDS:

Jua. Illnesses. Insecticide.

PAGES: 100
BIG AREA: Outra
AREA: Ciências Ambientais
SUMMARY:

The species Sarcomphalus joazeiro Mart. Popularly known as juá, juazeiro, juá-fruta and orange-de-vaqueiro, it is a plant endemic to the Caatinga of the Rhamnaceae family, but it is very diverse throughout the Brazilian Northeast. Urban pests, as published in resolution RDC 52 (Resolution of the Collegiate Board 52) are organisms that infest urban environments, which can cause damage to human health, domestic animals and economic losses. The uncontrolled use of insecticides can cause damage to the environment and human health, therefore, there is a ban on several products and more demanding regulations. With the aim of alleviating dependence on chemical insecticides, in communion with the urgency of finding new, safer, more ecological and efficient methods, one of the ways is the use of natural products such as plant extracts, which are being seen as an alternative and sustainable form of control. . The objective was to evaluate the potential bioinsecticide methanolic extract of Sacomphalus joazeiro Mart. on the Periplaneta americana and Aedes aegypti vectors. The bark of the stem of this plant was collected in the Muriti neighborhood in the municipality of Crato-CE. An exsicata of the species was collected at the location coordinates S 071°13.2.296' and W 039°22.602', identified with the number 15.146 and deposited at the Herbarium Carirense Dardano e Andrade Lima – HCDAL. The methanolic extract of the stem bark of S. joazeiro was produced in the rotaevoporator. P. americana cockroaches were collected using traps in a cemetery in Crato-CE and placed in a bucket for reproduction. Nymphs of Periplaneta americana were exposed to concentrations of 250, 200, 150, 100 and 50 mg/g and control with distilled water with food for diet for three days with a total of 30 cockroaches per concentration for 72 hours, after which they were selected 12 nymphs per concentration and behavioral evaluations were carried out and 6 nymphs per concentration for analysis of biochemical assays, based on oxidative stress markers and with the extract the antioxidant assay using the DPPH method with concentrations of 5 to 5000 µg/ml of the extract and 5 to 1000 µg/ml of the positive control with ascorbic acid, as well as repelling the nymphs using concentrations of 8, 4 and 2 mg/mL and the control with water for 4 hours. With the Aedes aegypti vector, eggs were collected using ovitraps in the Muriti neighborhood in Crato-CE and the larvicidal test was carried out with L3 larvae, at concentrations of 500, 250 and 125 µg/mL and a negative control with distilled water and a positive with pyriproxyfen in a B.O.D under suitable conditions for 120 hours and was done at LC50. The graphics were produced using Prism Software v.6. The methanolic extract presents some classes of secondary compounds, but does not present larvicidal activity at any of the concentrations tested against the vector. It does not present statistically significant antioxidant activity, however, it is clear that with increasing concentrations the extract may present moderate antioxidant activity. It presents repellent activity against P. americana at a concentration of 8 mg/mL, providing approximately 100% repellency throughout the four hours of exposure, however, it does not present insecticidal activity against P. americana. The extract does not show oxidative damage after evaluating protein and non-protein markers. The extract has the ability to reduce free iron levels at all concentrations tested and TBARS at a concentration of 500 mg/mL shows a moderate decrease. Thus, showing that the extract has the ability to reduce oxidative damage and, therefore, does not present damage and toxic effects on the P. americana vector. It is concluded that the methanolic extract does not present insecticidal activity for the two vectors of etiological agents, and does not cause oxidative damage, however, it presents repellent activity against P. americana.

BANKING MEMBERS:
Presidente - FRANCISCO ROBERTO DE AZEVEDO
Interna - ESTELITA LIMA CANDIDO
Externo à Instituição - Luiz Marivando Barros - URCA