Banca de QUALIFICAÇÃO: ANTONIO BARROS DE SOUZA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANTONIO BARROS DE SOUZA
DATA : 29/11/2019
HORA: 09:00
LOCAL: CAMPUS CRATO
TÍTULO:

ANTIBACTERIAN AND ANSIOLIC POTENTIAL OF THE ZANIPHUS JOAZEIRO LEAF ETHANOLIC EXTRACT

PALAVRAS-CHAVES:

Modulation; Zebrafish; Medicinal plants; Ziziphus Joazeiro

PÁGINAS: 76
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

Medicinal plants have been used since ancient times to treat various diseases and have subsidized scientific research in the discovery of new products richly active in pharmacological properties. In this context, the phytochemical prospection, the antimicrobial and modulatory activities of the antibiotics against multiresistant strains were evaluated, as well as the non-clinical safety profile by the open field test, toxicity, and finally, the anxiolytic activity and mechanisms of antimicrobial activity. action of ethanolic extract obtained from the leaves of the species Ziziphus joazeiro Mart., popularly known as juá. The ethanolic extract was obtained by maceration in P.A ethanol for 72 hours. Standard strains and clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were used and antibacterial activity to determine the minimum inhibitory concentration. The modulatory action was evaluated by the direct contact method using the antibiotics Gentamicin, Norfloxacin and Ampicillin against previously mentioned multiresistant bacterial species. The toxicity and anxiolytic activity test and its possible mechanisms used zebrafish as a model. The results obtained in phytochemical prospecting suggest the presence of metabolic components Flobabenic tannins, Leucoanthocyanidins, Flavonones, Flavonones, Catechins, Alcaloides, Steroids and triterpenoids. No other flavonoid subclasses were detected in this species. In the open field test, a decrease in locomotor capacity was observed when compared to diazepam, thus suggesting that the extract may have anxiolytic activity. The open field toxicity test showed one (LD50 ˃ 500 mg / kg), being preclinically safe in this model and therefore non-toxic. The extract has no clinically relevant antibacterial activity since a high value of minimal inhibitory concentration was obtained. However, it was effective in modulating the action of antibiotics against multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. In the anxiety model EtFoZj (10 or 100 or 500 mg / kg; ip) showed significantly similar anxiolytic effect (p> 0.05) similar to the effect of Diazepam (500 mg / kg; ip; p <0.01 vs Naive or vehicle). Given the anxiolytic activity demonstrated, the possible mechanisms of action for this activity were evaluated. Involvement with the following systems was tested: Gabaergic and serotonergic (5-HT2A, 5-HT1 and 5-HT2A / 2C, 5-HT3A / 3B). Through the results, it was possible to conclude that the extract is possibly acting in the same way as diazepam because, in the gabaergic test, this one suffered the antagonistic action of flumazenil. When the serotoninergic pathway was evaluated for all receptors tested, showing that its mechanism of action is also linked to serotonergic receptors. Therefore, this study adds new evidence and highlights the potential of Ziziphus joazeiro for the development of herbal medicines with anxiolytic and modulatory properties.

MEMBROS DA BANCA:
Interno - 2350490 - ESTELITA LIMA CANDIDO
Presidente - 1548701 - FRANCISCO ROBERTO DE AZEVEDO
Externo ao Programa - 2140426 - JACQUELINE COSMO ANDRADE PINHEIRO
Externo ao Programa - 2222214 - LAURA HEVILA INOCENCIO LEITE