Banca de DEFESA: ALYNE OLIVEIRA CORREIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALYNE OLIVEIRA CORREIA
DATE: 19/12/2024
TIME: 15:00
LOCAL: Sala de aula da Faculdade de Medicina - Famed
TITLE:

Evaluation of the effect of Dioclea violacea lectin in an experimental model of lipopolysaccharide-induced ventriculitis in rats

KEY WORDS:

Lipopolysaccharides. Brain damage. Dioclea violacea. Behavioral changes. Oxidative stress.

PAGES: 70
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Legume lectins are proteins of non-immunological origin with anti-inflammatory, antinociceptive, antidepressant and anti-ischemic activities. The lectin extracted from the seeds of Dioclea violacea (DVL) has been widely studied for these actions, in an attempt to broaden the therapeutic options for central nervous system (CNS) disorders. The administration of lipopolysaccharide (LPS) is often used to study diseases associated with CNS lesions in animals. LPS is an endotoxin from the outer membrane of Gram-negative bacteria. We therefore propose an experimental model of LPSinduced ventriculitis. Ventriculitis is defined as the infection of the cerebrospinal fluid in the ventricles of the brain caused by any microorganism. The aim of this study was to evaluate the effect of Dioclea violacea lectin in an experimental model of lipopolysaccharide-induced ventriculitis in rats. The study was approved by CEUA-FAMED/UFCA (Protocol No. 003/2024). Male Wistar rats (280-300 g) previously anesthetized (ketamine 90mg/kg and xylazine 10mg/kg, ip) were injected with LPS (12 μg/3 μL) into the right lateral ventricle by stereotactic surgery to induce ventriculitis. They were divided into four groups: Shan (Sh), Control (LPS+Saline), Injected with LPS with DVL treatment at concentrations of 0.25 and 0.5 μg/ μL. Twenty-four hours later, the animals were evaluated by behavioral tests (open field and forced swimming), followed by euthanasia to remove the brains for determination of antioxidant enzyme activity (superoxide dismutase-SOD and Catalase-CAT) and morphological study by triphenyltetrazolium chloride (TTC) staining. The results showed that LPS (Control: 17.8 ±1.4, n=5) increased the number of crossings (FO, 13.6±1.0, n=7) and intraventricular administration of DVL at concentrations of 0.25 μg/μL (8.80±2.8, n=5) and 0.5 μg/μL (12.3±1.1, n=7) were able to reverse this behavior. LPS caused a decrease in rearing and grooming behaviors, but treatment with DVL was unable to modify this behavior. In the forced swim test, animals injected with LPS treated with DVL at both concentrations (0.25 and 0.5 μg/μL) showed a 2.5-fold and 2.8-fold increase in immobility time, respectively, compared to the control group. LPS administration caused a significant increase (63.3%) in SOD activity in the control group (167.0±10.3, n=3) compared to the sham-operated group (94.1±11.3, n=4). Treatment with DVL at a concentration of 0.5 μg/μL was able to reverse this reduction (119.4±15.5, n=5). CAT quantification showed no significant change. TTC staining of the sections was able to identify a significant decrease in optical density in the control (117.3 ± 2.4, n=6) compared to the FO (140.7 ± 4.7, n=6). This finding was reversed in the animals treated with DVL 0.5 μg/μL (138.6 ± 5.8, n=6). Conclusion: LPS caused central damage in the proposed model, identified by behavioral changes and decreased neuronal viability, which were reversed by treatment with DVL.

COMMITTEE MEMBERS:
Externo à Instituição - GIOVANY MICHELY PINTO DA CRUZ
Interno - IRI SANDRO PAMPOLHA LIMA
Externo ao Programa - 1352200 - JOAO ANANIAS MACHADO FILHO - nullPresidente - MARIA ELIZABETH PEREIRA NOBRE