Banca de DEFESA: ADRIELLE RODRIGUES COSTA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ADRIELLE RODRIGUES COSTA
DATE: 04/04/2025
TIME: 08:30
LOCAL: CCAB
TITLE:

Vatairea macrocarpa lectin activity: antitumoral evaluation, anti -angiogenic effect and in vivo toxicity with Drosophila melanogaster

KEY WORDS:

VML. Cancer. Toxicity. Anticancer.

PAGES: 101
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Cancer is a multifactorial disease characterized by uncontrolled cell growth and a high mortality rate, making it one of the major global health challenges. Conventional therapies, such as chemotherapy, face significant limitations. In this context, lectins have demonstrated promising biological activities through their selective interaction with Tumor-Associated Carbohydrates (TACs) present on the surface of cancer cells, inhibiting processes such as cell proliferation, angiogenesis, and metastasis. This study investigates the lectin from Vatairea macrocarpa (VML), analyzing its cytotoxicity in human tumor cell lines, its antiangiogenic potential, and its toxicity in Drosophila melanogaster. After purification of the lectin, in vitro and in vivo assays were conducted to evaluate its biological properties and therapeutic potential. The results demonstrated that VML exhibited significant selective cytotoxicity in tumor cells. It inhibited leukemia cell lines, with IC50 values ranging from 3.5 µg/mL for HL-60 (acute promyelocytic leukemia) to 102.0 µg/mL for KG1 (acute myeloid leukemia). Additionally, it inhibited the growth of A549 lung cancer cells, with an IC50 of 97.21 µg/mL. However, it had no effect on other tumor cell lines and was not toxic to non-tumor cells such as HaCat and VERO. In the CAM model, VML exhibited a strong antiangiogenic effect, reducing A549-induced vascularization by 70.38% in a concentration-dependent manner, as well as inhibiting the angiogenic factors VEGF (71.07%) and TGF-β (65.97%). Immunohistochemistry confirmed the reduction in neovascularization and structural alterations in the chorioallantoic membrane, such as decreased fibroblasts and inflammatory cells. In the D. melanogaster model, VML showed no toxicity, suggesting that it is a promising molecule for in vivo studies. Given its ability to interact with specific carbohydrates on the cell surface, VML may contribute to selective antitumor effects, offering a potential alternative or complement to existing cancer therapies.

COMMITTEE MEMBERS:
Externo à Instituição - CLÉVER GOMES CARDOSO - UFG
Externa à Instituição - ANTONIA ELIENE DUARTE - URCA
Presidente - CLAUDENER SOUZA TEIXEIRA
Interno - IRI SANDRO PAMPOLHA LIMA
Externo à Instituição - JOSÉ WEVERTON ALMEIDA BEZERRA - URCA