Banca de DEFESA: CICERO JERFESSON FERREIRA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CICERO JERFESSON FERREIRA SILVA
DATE: 13/02/2025
TIME: 14:00
LOCAL: FAMED
TITLE:

EVALUATION OF THE SENSITIVITY OF CD1a PROTEIN IMMUNOHISTOCHEMISTRY AS AN AUXILIARY TOOL IN THE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS

 

KEY WORDS:

Leishmania braziliensis, Immunohistochemistry, CD1a marker

PAGES: 50
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

American tegumentary leishmaniasis (ATL) is an infectious, parasitic, vector-borne disease that manifests itself with skin and, occasionally, mucous membrane lesions, sometimes causing morphological and functional deformities. In addition to clinical elements, confirmation of the diagnosis requires additional tests, especially biopsy with histopathology. Identification of the parasite in histopathology is laborious, since the parasite is almost always scarce in the infected tissue and occasionally resembles other pathogenic microorganisms. Considering the need to optimize the identification of parasites in tissues with suspected ATL, an assay was designed using immunohistochemistry with the expression of the CD1a marker in dendritic cells as a possible histopathological tool in the diagnosis of ATL; accuracy study using 58 human skin samples, obtained by biopsy using punch no. 3, at the tropical pathology outpatient clinic of the Teaching Clinic of the Faculty of Medicine of the Federal University of Cariri, obtained between 2018 and 2024, with a confirmed diagnosis of ACL, based on clinical, epidemiological, routine histological, culture, PCR and therapeutic response. All samples were subjected to histological processing with paraffin inclusion and stained with hematoxylin/eosin (HE), culture using NNN medium, polymerase chain reaction in agarose gel and subjected to immunohistochemistry to evaluate the expression of the CD1a marker by the polymer methodology with peroxidase revelation. Of the 58 cases, (41.38%) were male, (24.14%) female, the youngest ages were between 10 and 19 years (12.07%) and the oldest ages were between 20 and 59 years (65.52%), (82.76%) from rural areas and (17.24%) from urban areas, approximately (41.38%) were farmers, (87.93%) literate, (1.72%) illiterate and (10.34%) did not respond. The parasite(s) or remains were observed in 30 patients in hematoxylin/eosin staining, only (3/17) cultures were positive and 2/25 samples were PCR positive; CD1a expression was observed in 56/58 samples. The calculation of the accuracy of the CD1a marker by immunohistochemistry for the diagnosis of ATL was (number of positive cases/58 x 100). It is therefore concluded that the use of CD1a immunostaining by immunohistochemistry can aid in the diagnosis, together with other techniques in skin biopsies of the edge of the ATL lesion.

 

COMMITTEE MEMBERS:
Presidente - CLAUDIO GLEIDISTON LIMA DA SILVA
Externa à Instituição - KELLY ROSE TAVARES NEVES - UFC
Interna - MARIA DO SOCORRO VIEIRA DOS SANTOS
Externo à Instituição - SAVIO SAMUEL FEITOSA MACHADO